Journal: BMC Genomics
Article Title: Analysis of the heat shock response in mouse liver reveals transcriptional dependence on the nuclear receptor peroxisome proliferator-activated receptor α (PPARα)
doi: 10.1186/1471-2164-11-16
Figure Lengend Snippet: Expression of Acox1 and Cyp4a proteins after WY and heat shock in livers and kidneys . A. Expression of ACO and Cyp4a in livers of wild-type or PPARα-null mice on control or WY diet, 24 hrs after HS or mock HS. Expression was assessed by Western blot using primary antibodies against the indicated proteins. B. Quantitation of the western blots in C. C. Altered protein expression in the kidneys of wild-type or PPARα-null mice on control or WY diet, 24 hrs after HS or mock HS. Expression was assessed by Western blot using primary antibodies against the indicated proteins.
Article Snippet: Immunoblots were developed using primary antibodies against acyl-CoA oxidase (ACO) (a gift from S. Alexson, Huddinge University Hospital, Huddinge, Sweden), HS proteins (Santa Cruz Biotechnology, Santa Cruz, CA; StressGen, Victoria, B.C., Canada) or CYP4A (GenTest, Waltham, MA) and appropriate secondary antibodies conjugated with horseradish peroxidase (Santa Cruz Biotechnology) in the presence of chemiluminescent substrate ECL (Amersham, Piscataway, NJ).
Techniques: Expressing, Control, Western Blot, Quantitation Assay